3.8.2. Study of pH Stability

The rTurgencin A freeze-dried fermentation supernatant was dissolved in a glycine solution at a pH of 2, a sodium acetate solution at a pH of 4, a Tris-HCl solution at a pH of 6, a Tris-HCl solution at a pH of 8, and a glycine solution at a pH of 10. The final concentrations of the buffer solution were all 100 mM. Then, the samples were treated at 37 °C for 4 h and subjected to the agar well diffusion assay. Twelve hours later, the inhibition circle was observed. The negative control was a buffer solution of different pHs.