4.1. DNA and Sample Preparation

The oligodeoxynucleotides used in this study were obtained from Eurofins Genomics (Tokyo, Japan) after purification to OPC grade or HPLC grade, which is only for fluorescent dye-attached DNA, and used without any further purification. The following DNA sequences were used for all the studies. Reconstructed IRDAptamer library: 5′-AGCGTCGAATACCACTACAC-AGGG-N6-TTAGGG-N6-TTAGGG-N6-TTAGGG-TAATGGAGCTCGTGGTCAGC-3′, forward primer: 5′- AGCGTCGAATACCACTACAC-3′, reverse primer: 5′-GCTGACCACGAGCTCCATTA-3′, biotinylated reverse primer: 5′-biotin-GCTGACCACGAGCTCCATTA-3′, MnG4C1: 5′-AGGG-GGGGAG-TTAGGG-CGCACG-TTAGGG-GTGCTA-TTAGGG-3′, MnG4C1-FT: 5′-FAM-AGGG-GGGGAG-TTAGGG-CGCACG-TTAGGG-GTGCTA-TTAGGG-TAMRA-3′ All other chemical reagents were obtained from Fuji-Film, Wako Pure Chemical Corporation and used without further purification.