Measurement of stool parameters

To collect pure stool and urine without any contamination, all ICR mice were bred in metabolic cages (Daejong Instrument Industry Co., LTD, Seoul, Korea) during the experimental period. The stool number and weight were measured as described in previous studies [9,17]. The stools excreted from each ICR mouse was collected at 10:00 am, after which stool samples were weighed three times per sample using an electric balance and the number of stools was counted three times. Also, the reduction rate of stool number was calculated using the following formula;

SV was represented stool number of Vehicle-treated group, while SL was indicated the stool number of Lop-treated group.

Furthermore, the stool moisture content was analyzed using the following formula;

A is indicated the weight of stools firstly collected after Lop administration, while B is represented the weight of stools after the dry at 60℃ for 12 hr.