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AnnexinV/PI apoptosis assay (flow cytometry)

SK Sadegh Khorrami
MD Manijeh Dogani
SM Saeed Esmaeili Mahani
MM Mehrdad Moosazadeh Moghaddam
RT Ramezan Ali Taheri
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Fluorescein isothiocyanate (FITC) and Propidium Iodide (PI)-conjugated annexin V intercalating agents were used to stain the cells and determine the apoptosis/necrosis rate of SH-SY5 cells. Briefly, SH-SY5 cells (2 × 105) were treated with Meth for 24 h following a 4-h pretreatment with the indicated concentration of the extract or AgNPs. After detachment of the incubated cells, they were resuspended in 100 μL of 1 × binding buffer, including 20 μg/ml of Annexin V and 1 μg/mL of PI for 15 min at RT. These cells were finally assessed on a BD FACSVerse (CyFlow Space, Sysmex, Germany).

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