Single-nucleus transcriptome sequencing using snRNA-seq

JH John W. Hickey
WB Winston R. Becker
SN Stephanie A. Nevins
AH Aaron Horning
AP Almudena Espin Perez
CZ Chenchen Zhu
BZ Bokai Zhu
BW Bei Wei
RC Roxanne Chiu
DC Derek C. Chen
DC Daniel L. Cotter
EE Edward D. Esplin
AW Annika K. Weimer
CC Chiara Caraccio
VV Vishal Venkataraaman
CS Christian M. Schürch
SB Sarah Black
MB Maria Brbić
KC Kaidi Cao
SC Shuxiao Chen
WZ Weiruo Zhang
EM Emma Monte
NZ Nancy R. Zhang
ZM Zongming Ma
JL Jure Leskovec
ZZ Zhengyan Zhang
SL Shin Lin
TL Teri Longacre
SP Sylvia K. Plevritis
YL Yiing Lin
GN Garry P. Nolan
WG William J. Greenleaf
MS Michael Snyder
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snRNA-seq targeting 9,000 nuclei per sample was performed using Chromium Next GEM Single Cell 3’ Reagent Kits v3.1 (10x Genomics, 1000121) and the Chromium Next GEM Chip G Single Cell Kit (10x Genomics, 1000120). Libraries were pooled and sequenced on the Illumina NovaSeq 6000 system (read 1 = 28 bp, i7 index = 8 bp, i5 index = 0 bp, read 2 = 91 bp read configuration) targeting an average of 20,000 reads per nucleus.

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