snRNA-seq targeting 9,000 nuclei per sample was performed using Chromium Next GEM Single Cell 3’ Reagent Kits v3.1 (10x Genomics, 1000121) and the Chromium Next GEM Chip G Single Cell Kit (10x Genomics, 1000120). Libraries were pooled and sequenced on the Illumina NovaSeq 6000 system (read 1 = 28 bp, i7 index = 8 bp, i5 index = 0 bp, read 2 = 91 bp read configuration) targeting an average of 20,000 reads per nucleus.
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