The material for analysis corresponded to the stored remnants of nasopharyngeal swabs that had been taken for diagnostic purposes. RNA was purified from 300 μL of nasopharyngeal exudate in a KingFisher (Thermo Fisher Scientific) equipment. Cepheid Xpert Xpress SARS-CoV-2 Assay (Cepheid, CA) and TaqPath COVID-19 CE-IVD RT-PCR kit (Thermo Fisher Scientific) were applied for SARS-CoV-2 RT-PCR based detection as per the manufacturer’s instructions.
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