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EDTA-treated human blood was incubated in microtitre wells containing either S. aureus USA300 biofilm, 5HE-C14-TMA (at 256 mg/L) only or biofilm with 5HE-C14-TMA (at 256 mg/L) and incubated for 2 h at 37 °C. Plasma samples were collected by centrifugation (1000 g) and assayed for human cytokines using a Bio-Plex 200 (Bio-Rad). A standard curve was employed to maximize sensitivity for samples containing low cytokine levels. At least three different donor blood samples (with three replicates for each) were used.
Copyright and License information: ©2016 The Author 2016. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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