A microwave-assisted acid digestion method is necessary to destroy the seaweed matrix for total Ti determination by ICP-MS. Seaweed samples were digested with a mixture of nitric acid and hydrogen peroxide following the method described in a previous work [28]. The digested samples were diluted with ultrapure water (dilutions 1:10 to 1:100) until 25 mL before their analysis by ICP-MS. This procedure was applied in triplicate to each sample, and two blanks were prepared for each set of experiments.
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