Formalin-fixed, paraffin-embedded (FFPE) tissue microarrays (TMA) composed of meningioma tumor probes were cut in 2 µm sections and stained using the BOND Fully Automated IHC Staining System or Ventana Roche BenchMark ULTRA Fully Automated IHC Staining System. The sections were incubated with primary antibodies against CD4 (Ventana-Roche, Order No. 790-4423/05552737001, prediluted) and CD8 (DAKO A/S, Order No. M71030, dilution: 1:100). For the detection of T regulatory cells, antibodies were first applied against FOXP3 (Abcam Limited, Order No. ab2003, dilution 1:150) and then against CD3 (Thermo Scientific, Order no. MA1-90,582, dilution: 1:100). Visualization of the antibodies was performed with the VENTANA BenchMark ULTRA, the DF AP or DAB kit. All sections were counterstained with hematoxylin.
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