T-cell mapping

GM Gioele Medici
LF Lena K. Freudenmann
JV Julia Velz
SW Sophie Shih-Yüng Wang
KK Konstantina Kapolou
NP Nagarajan Paramasivam
LM Lena Mühlenbruch
DK Daniel J. Kowalewski
FV Flavio Vasella
TB Tatjana Bilich
BF Beat M. Frey
MD Marissa L. Dubbelaar
AP Angelica Brooke Patterson
AZ Anna Maria Zeitlberger
MS Manuela Silginer
PR Patrick Roth
TW Tobias Weiss
HW Hans-Georg Wirsching
NK Niklaus Krayenbühl
OB Oliver Bozinov
LR Luca Regli
HR Hans-Georg Rammensee
ER Elisabeth Jane Rushing
FS Felix Sahm
JW Juliane S. Walz
MW Michael Weller
MN Marian C. Neidert
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Formalin-fixed, paraffin-embedded (FFPE) tissue microarrays (TMA) composed of meningioma tumor probes were cut in 2 µm sections and stained using the BOND Fully Automated IHC Staining System or Ventana Roche BenchMark ULTRA Fully Automated IHC Staining System. The sections were incubated with primary antibodies against CD4 (Ventana-Roche, Order No. 790-4423/05552737001, prediluted) and CD8 (DAKO A/S, Order No. M71030, dilution: 1:100). For the detection of T regulatory cells, antibodies were first applied against FOXP3 (Abcam Limited, Order No. ab2003, dilution 1:150) and then against CD3 (Thermo Scientific, Order no. MA1-90,582, dilution: 1:100). Visualization of the antibodies was performed with the VENTANA BenchMark ULTRA, the DF AP or DAB kit. All sections were counterstained with hematoxylin.

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