Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
The cell suspension was prepared at a final density of 6 × 104 cells/well. After washing with PBS that had been pre-cooled at 4 °C, 2 μl Annexin V-APC and PI (Thermo Fisher Scientific, USA) were added for staining for 20 min in dark, and then the extent of apoptosis was assessed by flow cytometric analysis (MilliporeSigma, USA). These experiments were performed in triplicate. Bar graphs are the mean ± SD of three separate experiments.
Copyright and License information: The Author(s) ©2023
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
