Graph creation and statistical analysis were performed using GraphPad Prism for Windows (GraphPad Software, Inc.), version 9.0. Results are presented as means ± standard error of the mean. Normality of data was tested using the Shapiro–Wilk test. Data not showing normal distribution were transformed before applying the parametric ANOVA. Statistical significance was assessed using t-test or when multiple groups were analysed simultaneously, one-way ANOVA followed by Tukey’s multiple comparison post hoc test, two-way ANOVA followed by Sidak’s post hoc test, or three-way ANOVA followed by Sidak’s post hoc test. Significance was represented as follows: *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
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