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Immunoblotting

CI Consuelo Ibar
KC Krishna Chinthalapudi
SH Sarah M Heissler
KI Kenneth D Irvine
ME Michael B Eisen
A Anonymous
A Anonymous
CI Consuelo Ibar
KC Krishna Chinthalapudi
SH Sarah M Heissler
KI Kenneth D Irvine
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Wing discs (20 discs per lane) were lysed in 2× Laemmli Sample Buffer (Bio-Rad, #1610737) supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail (Calbiochem). Protein samples were loaded in 4–15% gradient gels (Bio-Rad). Antibodies used for immunoblotting include mouse anti-GFP (1:1000; Cell Signaling Technology, #2955, RRID:AB_1196614) and as a loading control rabbit anti-GAPDH (1:5000; Santa Cruz Biotechnology, sc-25778, RRID:AB_10167668). Blots were visualized with fluorescent-conjugated secondary antibodies (LI-COR Biosciences) and the Odyssey Imaging System (LI-COR Biosciences).

Copyright and License information:  ©2023, Ibar et al
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