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Generation of H3‐mCherry and H3T3A‐mCherry transgenes
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The histone sequences, such as H3‐mCherry and H3T3A‐mCherry, were recovered as an XbaI‐flanked fragment and were subsequently inserted into the XbaI site of the UASp to generate: UASp‐H3‐mCherry and UASp‐H3T3A‐mCherry plasmids, which were introduced to w1118 flies by P‐element‐mediated germline transformation (Bestgene, Inc.).
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