Luciferase assays

Segments surrounding rs1663689 and the promoter of ADGRG6 were cloned into the multiple cloning site of the pGL3‐basic vector and verified by Sanger sequencing. 293 T cells were cultured in 10% FBS‐DMEM until ready for transfection. Luciferase plasmids were cotransfected into 293 T cells with the Renilla plasmid pRL‐TK as a control using Lipofectamine 3000 (1684916, Thermo Fisher). After 24 h, firefly and Renilla luciferase activities were measured using the Dual‐Luciferase Reporter Assay System (E1960, Promega) according to the manufacturer's protocol.