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The number of animals used throughout the study was n=6 for mechanical pressure, where one or more A-fibers were recorded from each mouse. Before surgery, mice were sacrificed by CO2 inhalation, followed by cervical dislocation. The hair on the posterior leg was shaved, and the foot was fixed on a linen pad. The hairy epidermis of the hind paw innervated by the saphenous or sural nerve was dissected after the hair on the leg was clipped. Attached connective tissue, muscle, and tendon were removed.
Copyright and License information: Experimental Neurobiology ©2023
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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