Animal husbandry

Foxc1 ^fl/fl , Foxc2 ^fl/fl , Foxc1 ^fl/fl ;Foxc2 ^fl/fl (Sasman et al, ²⁰¹²), Cdh5‐Cre ^ERT2 (Sorensen et al, ²⁰⁰⁹), Vegfr3‐Cre ^ERT2 (Martinez‐Corral et al, ²⁰¹⁶), and Foxc2‐Cre ^ERT2 (Amin et al, ²⁰¹⁷) mice were used. EC‐specific or LEC‐specific Foxc1, Foxc2, and compound Foxc1;Foxc2‐mutant mice were generated by crossing Foxc‐floxed females (Foxc1 ^fl/fl , Foxc2 ^fl/fl , and Foxc1 ^fl/fl ;Foxc2 ^fl/fl ) with Cdh5‐Cre ^ERT2 ;Foxc1 ^fl/fl (EC‐Foxc1‐KO), Cdh5‐Cre ^ERT2 ;Foxc2 ^fl/fl (EC‐Foxc2‐KO), Cdh5‐Cre ^ERT2 ;Foxc1 ^fl/fl ;Foxc2 ^fl/fl (EC‐Foxc‐DKO), Vegfr3‐Cre ^ERT2 ;Foxc1 ^fl/fl (LEC‐Foxc1‐KO), Vegfr3‐Cre ^ERT2 ;Foxc2 ^fl/fl (LEC‐Foxc2‐KO), Vegfr3‐Cre ^ERT2 ;Foxc1 ^fl/fl ;Foxc2 ^fl/fl (LEC‐Foxc‐DKO) males, respectively, as described previously (Norden et al, ²⁰²⁰). For Cre recombination efficiency detection, mTmG/+;Cdh5‐Cre ^ERT2 ;Foxc1 ^fl/fl ;Foxc2 ^fl/fl (mTmG/EC‐Foxc‐DKO), mTmG/+;Vegfr3‐Cre ^ERT2 ;Foxc1 ^fl/fl ;Foxc2 ^fl/fl (mTmG/LEC‐Foxc‐DKO) and mTmG/+;Foxc2‐Cre ^ERT2 mice were generated by crossing mTmG females (mTmG/mTmG;Foxc1 ^fl/fl ;Foxc2 ^fl/fl and mTmG/mTmG) with EC‐Foxc‐DKO, LEC‐Foxc‐DKO and Foxc2‐Cre ^ERT2 males, respectively. Genotyping of mice was performed by Transnetyx Inc.