Dual luciferase reporter assay

The CDSs of each PrMYB and PrbHLH were cloned and ligated into the pSN1301 vector to construct effectors, and the promoters of PrF3H, PrDFR, and PrANS were ligated into pGreenII-0800-LUC vector to generate reporters. The paired effector and reporter were co-transfected into N. benthamiana leaves using the Agrobacterium-mediated approach [88, 89]. LUC and REN activities were analyzed using the Dual Luciferase^® Reporter Assay System (Promega, USA) and a GloMax^® 20/20 Luminometer (Promega, USA). The luciferase complementation imaging assay was performed using the Tanon 5200 Multi Imaging System (Tanon, China). The primers used in our LUC assays are listed in Supplementary Data Table S4.