2.2. Size-exclusion chromatography analysis of CmnB under an acidic condition

Shu-Ing Toh; Chieh-Ling Lo; Chin-Yuan Chang

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2.2. Size-exclusion chromatography analysis of CmnB under an acidic condition

ST Shu-Ing Toh

CL Chieh-Ling Lo

CC Chin-Yuan Chang

This method is extracted from research article: Acta Crystallogr F Struct Biol Commun, Jul 2023

Crystal structure of CmnB involved in the biosynthesis of the nonproteinogenic amino acid l-2,3-diaminopropionic acid

DOI: 10.1107/S2053230X23005769

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Size-exclusion chromatography was performed using a HiLoad Superdex 16/60 pg column (Cytiva) with an NGC Chromatography System (Bio-Rad) at 4°C with 1 ml of sample loaded per run. The column was calibrated with Gel Filtration Standard (Bio-Rad) and developed with elution buffer (100 mM citrate, 200 mM Na₂HPO₄ pH 5.5) at a flow rate of 1 ml min⁻¹.

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