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Cytokine IL-2 was measured by Bio-plex Pro Human Cytokine IL-2 Assays (Bio-Rad, Hercules, CA, USA). The median fluorescent intensity of the IL-2 bead set was measured on a Bio-plex 200 instrument (Bio-Rad). Cytokine concentration was calculated from a control curve of standard provided separately by the same supplier. In the absence of a response to the peptides, the amount of cytokine was considered as below the LLOD and a value equal to the lowest value on the standard curve was given for that sample.
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