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AV Aina Vaivade
AW Anna Wiberg
PK Payam Emami Khoonsari
HC Henrik Carlsson
SH Stephanie Herman
AA Asma Al-Grety
EF Eva Freyhult
UO Ulla Olsson-Strömberg
JB Joachim Burman
KK Kim Kultima
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Peripheral blood samples were collected from 16 RRMS patients treated with AHSCT at Uppsala University Hospital, Sweden, from 2015 to 2016. The procedure used for stem cell collection and transplantation has been described previously by Wiberg et al. 2020. In brief, the peripheral blood samples were collected at ten timepoints (Fig. 1): (T1) prior to mobilization, (T2) following mobilization with cyclophosphamide, (T3) timepoint of stem cell collection, (T4) prior to the conditioning regime with cyclophosphamide and thymogobuline, (T5) prior to stem cell reinfusion (SCR), (T6) + 1 day after SCR, (T7) + 3 days after SCR, (T8) + 5 days after SCR, (T9) at the time of hospital discharge and (T10) at the three-month follow-up. After centrifuging the whole blood samples at 2400 g for seven minutes and allowing them to coagulate, the serum was stored at -80℃ in sterile polypropylene tubes.

Timeline for AHSCT, including the ten-time points for blood sampling. (T1) prior to mobilization, (T2) following mobilization using cyclophosphamide, (T3) stem cell collection, (T4) prior to conditioning, (T5) prior to SCR, (T6) + 1 day after SCR, (T7) + 3 days after SCR, (T8) + 5 days after SCR, (T9) hospital discharge and (T10) the three-month follow-up

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