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2.3. Packed cell volume and hematocrit concentration technique
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The PCV value of the blood samples was determined using capillary tubes with heparin (TECNAN, Navarra, Spain). The tubes were centrifuged (TG12M Madell Technology Corporation, Riverside, California, USA) at 12.000 rpm for 5 min. To evidence the parasite in the blood, within the first 4 h of sample collection, the heparinized capillary tubes were observed under a microscope (KRUSS, Hamburg, Germany) according to the HCT technique (28).
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