Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Quantitative real-time PCR (qRT-PCR)
Ask a
question
Favorite
Total cellular RNA was extracted using Trizol (15,596,026, Thermo, USA). The cDNA reverse transcription kit (4,368,814, Invitrogen, USA) was used to convert RNA into complementary DNAs (cDNAs). Gene expression analysis was performed on the ABI 7900 system using SYBR Green qPCR Mix (HY-K0501A, MedChemExpress, USA). Using β-actin or U6 as an internal reference, gene level was calculated by 2−ΔΔCt method. The primers sequences used were as follows:
Copyright and License information: The Author(s) ©2023
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
