Cell culture

The MCF10A (cat. no. CL-0525) cell line was purchased from Wuhan Procell Company, derived from human normal mammary epithelial cells, and was cultured in specialized MCF10A medium (Procell Life Science & Technology Co., Ltd.). The human BC cell lines, SKBR3 (cat. no. 1101HUM-PUMC000085), MCF7 (cat. no. 1101HUM-PUMC000013) and Hs578T (cat. no. 1101HUM-PUMC000670), were cultured in DMEM (Dalian Meilun Biology Technology Co.) supplemented with 10% fetal bovine serum (Shanghai ExCell Biological Products Co., Ltd.), 100 U/ml penicillin and 100 µg/ml streptomycin (Dalian Meilun Biology Technology Co., Ltd.) in a 5% CO₂ incubator at 37°C, while the MDA-MB-231 (cat. no. 1101HUM-PUMC000014) and MDA-MB-468 cells (cat. no. 1101HUM-PUMC000249) were cultured in RPMI-1640 medium (Dalian Meilun Biology Technology Co.). All the human BC cell lines were purchased from the ChineseNational Infrastructure of Cell line Resource (NICR).