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2.15. RNA-immunoprecipitation (RIP)
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RIP reactions were carried out using Magna-RIP RNA-IP Kit (Millipore, USA) with normal rabbit IgG control, anti-rabbit-HuR or anti-rabbit-TTP antibodies according to the manufacturer's instructions. RNA was extracted using TRIzol, qPCR and qRT-PCR were performed as described above, and the % input method was used to calculate RNA enrichment.
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