Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.3. Construction of a ceRNA regulatory network

YG Yilin Guo
LW Lu Wang
ZX Zhen Xu
ML Mengqi Li
WW Wuliang Wang
YB Yangyang Bai
XX Xingyue Xu
RL Rui Li
HZ Hu Zhao
request Request a Protocol
ask Ask a question
Favorite

To investigate the potential existence of DEGs involved in a competing endogenous RNA (ceRNA) network mediated by differentially expressed lncRNA and miRNA, we conducted miRanda (http://www.microrna.org/), miRWalk (http://129.206.7.150/), and Targetscan (http://www.targetscan.org/) analyses to predict miRNA-mRNA interactions, while StarBase (https://starbase.sysu.edu.cn/) was used to predict lncRNA-miRNA interactions. The resulting interactions were then visualized using Cytoscape software (version 3.9.1).

Copyright and License information:  ©2023 Guo, Wang, Xu, Li, Wang, Bai, Xu, Li and Zhao
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A