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cDNA prepared in a reverse-transcription reaction was used as template for real-time PCR analysis (ABI 7500—Applied Biosystem) using a miScript SYBR Green Kit (Qiagen). In details, miScript miRNA PCR Array Human miFinder was used (MIHS-001Z), in 96-well format to perform a preliminary microRNA profiling. The consequent miRNAs expression was than confirmed using real-time PCR analysis (ABI 7500—Applied Biosystem) and a miScript miRNA PCR Primers (Qiagen). The miRNAs’ expression was normalized to the expression of Hs_RNU6-2.
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