2.10. Statistical analysis

Control and ionomycin live-imaging quantification data were evaluated separately through analysis of variance (ANOVA), followed by post hoc Tukey test of each nucleus type over time using R.

For proteomic analyses of nucleus and secretome, as well as the analysis of citrullinated peptides, the comparisons between groups (control and ionomycin) in each fraction was performed with an unpaired t-test. The P-value was adjusted for multiple comparisons applying an FDR of 0.05. These analyses were performed in R and Perseus.

For percentages in each fraction, after a t-test, proteins with adjusted P-value < 0.05 and with mean ratio of ionomycin/control >2 or <0.5 in at least one fraction each, were considered significantly different.