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Nitric oxide radical scavenging activity

MM Manal G. Mahmoud
MA Mohamed E. El Awady
MS Manal S. Selim
AI Abeer Y. Ibrahim
FI Faten M. Ibrahim
SM Sahar S. Mohamed
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Using sodium nitroprusside (SNP), the NO· radical scavenging activity of tested material was determined and was compared with two standard materials: BHT and Ascorbic Acid. NO· was generated from SNP in an aqueous solution at physiological pH to produce nitrite ions which were measured by the Greiss reagent [42]. Greiss reagent constitutes 1% sulfanilamide in 5% ortho-H3PO4 and 0.1% naphthyl ethylene diamine dihydrochloride. The reaction mixture contained from 2 ml of polysaccharide and standard compounds at different concentrations and SNP (10 mM) in phosphate-buffered saline pH 7.4 were incubated at 25 °C for 150 min. After incubation, 1-ml samples of reaction mixtures were removed and were diluted with 1 ml Greiss reagent. The absorbance of these solutions was measured at 540 nm against the corresponding blank solution.

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