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The nucleosome samples were prepared as previously described.8 For nucleosomal DNA, a 147 bp human α-satellite sequence with a single-strand 20-base linker with a biotin at its 5′ end was prepared by ligating six fragments in which the 54th nucleotide of the Watson (forward) strand was replaced with Cy3 (Integrated DNA Technology Inc., Coralville, IA). See Figure S1 for details on the DNA sequence and the labeling position.
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