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Paraformaldehyde (4%) was used to fix 20 mM NaHS-treated cells and control cells for 10 min. The cells were then stained with 5 mg/L Hoechst 33258 for 10 min, after which they were observed under an Olympus fluorescent microscope (Tokyo, Japan). Hoechst 33258 (H266330, Aladdin, Shanghai, China) staining revealed changes in chromatin morphology. The apoptotic cell nuclei showed condensed, fractured fluorescence representing chromosomal condensation and fragmentation, while normal cells exhibited uniform fluorescence and a completely rounded nucleus.
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