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Following a 48h co-culture of lymphocytes with OE33 P and OE33 R tumour cells, the viability of tumour cells was assessed using a cell counting kit (CCK-8, Sigma, USA) viability assay. 5 µl of CCK-8 solution was added to each well for 1h at 37°CC, 5% CO2. The optical density was measured using the Versa Max microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm and 650 nm (reference wavelength). Formula: (viability OE33 cell-lymphocyte co-culture)-(viability PBMCs alone)/(viability untreated OE33 cells alone) × 100 = % live cells.
Copyright and License information: ©2023 Davern, Gaughan, O’ Connell, Moran, Mylod, Sheppard, Ramjit, Yun-Tong Kung, Phelan, Davey, Ryan, Butler, Quinn, Howard, Tone, Phoenix, Butt, Lynam-Lennon, Maher, Ravi, Donohoe, Reynolds, Lysaght and Donlon
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