Yeast two-hybrid (Y2H) assays

BW Bingsen Wang
WH Wenfeng He
MH Mengshu Huang
JF Jiachen Feng
YL Yanping Li
LY Liu Yu
YW Yuqi Wang
DZ Dan Zhou
CM Chengzhen Meng
DC Dong Cheng
NT Ning Tang
BS Botao Song
HC Huilan Chen
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The full-length coding sequences were cloned into the pGADT7 vector or the pGBKT7 vector. The primers of related genes were listed in Table S1 (see online supplementary material). Target combinations were transformed into AH109 yeast and incubated on selective SD/−Leu/−Trp/-His/−Ade medium with Aureobasidin A (AbA) and X-α-gal.

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