2.5. Bacterial DNA extraction and WGS

All isolates were cultured on general nutrient agar plates at 36 ± 1°C for 24 h. DNA was extracted with a HiPurA Bacterial DNA kit (Magen, Guangzhou, China) based on the manufacturer's instructions. The extracted DNA was measured for DNA concentration using a NanoDrop ultraviolet (UV)-visible spectrophotometer. The required concentration was >20 ng/μl, with a total amount of 1–2 μg. The obtained DNA was used for WGS. DNA-constructed paired-end libraries were generated for WGS (Illumina HiSeq, San Diego, CA, United States) with a target coverage of 100× and 300 bp length. The quality of the raw reads was assessed with FastQC 0.11.9 (Andrews, 2010). Kraken 2 was used to classify and identify the QC sequence (Wood et al., 2019). Raw reads were assembled with SPAdes (version 3.14) with read error correction enabled (Bankevich et al., 2012).