Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Genetic diversity analysis

AK Arun Kumar
UR Upashna Rai
BR Bhupen Roka
AJ Alankar K. Jha
PR P. Anuradha Reddy
request Request a Protocol
ask Ask a question
Favorite

Consensus genotypes were used to determine overall expected (HE) and observed (HO) heterozygosities using allele frequency function in CERVUS 3.0. Polymorphism Information Content (PIC) of heterozygous loci was also obtained from allele frequencies. Alleles deviating from Hardy–Weinberg equilibrium (HWE) were tested with Genepop v4 (Rousset 2008) with default Markov chain parameters. Linkage disequilibrium (LD) estimates between loci and inbreeding coefficient (FIS) in the population were determined using FSTAT (Goudet 1995).

Copyright and License information: The Author(s) ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A