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PCR was performed on all LA-MRSA strains to detect the presence of the mecA and a specific sequence of sau1-hsdS1 gene, markers for methicillin resistance, and CC398, respectively, as previously described [16,17]. S. aureus ATCC 43300 and S. aureus ATCC 29213 were used as mecA positive and negative controls, respectively.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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