Analysis of Ubow mice

LK Lukas Kaltenbach
PM Paloma Martzloff
SB Sarah K. Bambach
NA Nadim Aizarani
MM Michael Mihlan
AG Alina Gavrilov
KG Katharina M. Glaser
MS Manuel Stecher
RT Roland Thünauer
AT Aude Thiriot
KH Klaus Heger
KK Katrin Kierdorf
SW Stephan Wienert
UA Ulrich H. von Andrian
MS Marc Schmidt-Supprian
CN Claus Nerlov
FK Frederick Klauschen
AR Axel Roers
MB Marc Bajénoff
DG Dominic Grün
TL Tim Lämmermann
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To quantify MC cluster formation in Ubow mice, ear skin whole mounts were imaged using a LSM780 (Zeiss) confocal microscope equipped with a Plan-Apochromat ×20 M27 objective (Zeiss). Z stacks of 25 to 42 µm with a 2-µm step size and multiple tiles were acquired using ZEN black software, and 2 × 2 tiled images (1.2 × 1.2 mm; covering a minimum of 600 MCs) were stitched using ZEN blue software. Statistical analysis and quantification of spatial MC distribution were performed using ClusterQuant2D software21,60. Detailed information of the analysis is provided in Supplementary Note 5.

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