Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Preparation of female-conditioned water

TN Tomoaki Nakada
FT Fumiyo Toyoda
KM Kouhei Matsuda
TN Takashi Nakakura
IH Itaru Hasunuma
KY Kazutoshi Yamamoto
SO Satomi Onoue
MY Makoto Yokosuka
SK Sakae Kikuyama
request Request a Protocol
ask Ask a question
Favorite

Eight sexually developed females were kept in a container filled with 800 mL tap water for 48 hours. The water was then filtered using Whatman filter paper and the filtrate was lyophilised. The lyophilised sample was dissolved in 50 mM Tris-HCl (8.5 mL) containing 150 mM NaCl (pH 7.5) and filtered again using 0.45-μm-pore filters (DISMIC-25CS, Advantec). One quarter of the total amount of water was saved while the remainder was used for affinity chromatography.

Copyright and License information:  ©2017, The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A