Generation of HEK293T cell line for IRF7 nuclear binding studies.

HEK293T cells were purchased from Takara and grown in DMEM containing 10% FBS and antibiotics as per the manufacturer’s protocol in a humidified incubator containing 5% CO₂ at 37°C. HEK293T cells were used to create a stable cell line for the WT and mut IRF7 plasmids. Briefly, the lentiviral vector and packing vectors psPAX2 and pMD2.G were transfected into HEK293T cells using calcium phosphate transfection to produce lentiviral particles (42). After 2 days of transfection, supernatant containing lentiviral particles was harvested and used to transduce HEK293T cells to generate the stable cell lines 293T/Ø-dTomato, 293T/IRF7-FLAG-dTomato, and 293T/IRF7mut-FLAG-dTomato with comparable expression levels (Supplemental Figure 2).