To identify the size distribution and concentration of isolated particles, a NanoSight LM10 system (Malvern Instruments Ltd. Malvern, UK) was used to detect the diluted exosomes. First, the sample detection slot was cleaned with Duchenne phosphate buffer without any nanoparticles. Then the exosome samples were diluted with Duchenne phosphate buffer 10 times and thoroughly mixed. The concentration and particle size of exosomes samples were determined by the NanoSight LM10 system software.
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