The plasmid pLZ009 -GFP-NRPE1 was constructed by inserting the DNA sequence encoding Arabidopsis thaliana NRPE1 (At2g40030) by a homogenous recombination method (pEASY®-Basic Seamless Cloning and Assembly Kit, Transgen Biotech, Inc) into a modified pLZ009 vector (pLZ009-GFP), which contains the CaMV35S promoter, the NOS terminator, and a GFP-expressing cassette consisting of the UBQ10 promoter, the coding sequence of GFP with the N7 nuclear localization signal at its N-terminus, and the UBQ10 terminator. The plasmid pETDuet1-KTF1-SPT4 was constructed by inserting the coding region of KTF1 (residues 1–712; At5g04290) and SPT4 (At5g63670) into modified pETDuet1 vector (Novagen), which contains an 6xHis tagged SUMOstar51.
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