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M6A immunoprecipitation (MeRIP) and RNA immunoprecipitation (RIP) assay

ZZ Zhiyang Zhou
YC Yukun Cao
YY Yuan Yang
SW Shouman Wang
FC Feiyu Chen
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MeRIP and RIP were used to validate the interaction between METTL3 and lnc KCNQ1OT1. A BersinBio RIP assay kit (BersinBio, Guangzhou, China) and m6A RNA Enrichment Kit (EPIGENTEK, Farmingdale, NY, USA) were conducted following the manufacturer’s protocol. Cell lysates were incubated with magnetic beads and anti-m6A (Abcam) or anti-METTL3 (Abcam) or anti-AGO2 (Abcam) or anti-IgG (Abcam). Lnc KCNQ1OT1 with m6A modification, lnc KCNQ1OT1, and miR-103a-3p enrichment were evaluated by RT-qPCR.

Copyright and License information:  ©2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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