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2.4. Histopathologic Examination
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Sections from bone and cartilage were fixed in 10% formalin for 12 h, then decalcified in formic acid. The sections were processed in an automated tissue process (Leica, ASP 300S, Leica Biosystems, Richmond, IL, USA) and were paraffin embedded using a paraffin embedding system. Four-micrometer-thick sections were cut, and they were prepared and stained with hematoxylin and eosin stain (H&E) via an automated slide stainer system (Leica ST5010) for histopathological examination. Sections were examined under a light microscope.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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