2.7. Effect of Yeast Concentration on Biocontrol Efficacy of Botrytis cinerea In Vivo

To evaluate the effect of the concentration of the yeast strains selected for their ability to biocontrol grey mold, wounded berries were submerged in a yeast suspension at concentrations of 1 × 10⁷, 1 × 10⁸ or 1 × 10⁹ cells mL⁻¹ in accordance with each treatment. Twenty-four hours later, they were sprayed with a conidial suspension of the pathogen at a concentration of 1 × 10⁴ spores mL⁻¹.

After 7 days at 20 °C, rot incidence was recorded according to Vero et al. [37]. Three replicates were established for each treatment in a completely randomized design in which each replicate was made up of 28 berries and the entire experiment was repeated twice.