All specimens were scanned using micro-CT (SCANCO MEDICAL AG, Switzerland) with a resolution of 10 μm to evaluate neurovascular and new bone formation. Then, 3D images were reconstructed using 3D Creator software (GE Healthcare BioSciences, Chalfont-St. Giles, UK). Blood vessels and bone tissues were separately reconstructed according to different grey value ranges. A region corresponding to vessels perfused with microfil on the micro-CT grey scale range was selected, and the parameters of vessel number (VN) and vessel volume (VV) were analysed. Three-dimensional measurements of the amounts of bone volume per total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th) and trabecular spacing (Tb.Sp) in the bone tissue were also calculated by microCT.
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