2.6. Cardiotoxin injury‐induced skeletal muscle damage in mice

Skeletal muscle injury was performed as described previously. ¹⁷ Briefly, C57BL/6J male mice (16 months old) were randomly divided into an uninjured group treated with phosphate buffered saline (PBS) (referred to Sham group), and the injured group treated with PBS (referred to post‐injury‐Vehicle group) or recombinant SESN1 protein (referred to post‐injury‐rSESN1 group). For the injured group, 10 μM of cardiotoxin (CTX, Latoxan) in 25 or 50 μL PBS was injected twice into tibialis anterior muscle or quadriceps muscle, respectively. In the following 7 days, recombinant SESN1 protein (0.5 μg/mL) in 30 or 60 μL PBS was injected into tibialis anterior muscle or quadriceps muscle, respectively.