Immunohistochemistry and lysosensor green stain

The cell climbing sheets were washed three times with PBS after administration, and were fixed with 4% paraformaldehyde for 30 min; then soaked in 0.1% Triton X100 (G1204, Servicebio, Beijing, China) for 5 min, and 5% BSA ({"type":"entrez-nucleotide","attrs":{"text":"GC305006","term_id":"196798989","term_text":"GC305006"}}GC305006, Servicebio, Wuhan, China) blocked the antigen, the primary antibody is incubated overnight at 4 °C. The cell climbing sheets are washed three times with PBS, and then incubated with CY3 or FITC conjugated secondary antibody (GB21301/GB22301, Servicebio, Wuhan, China) for 1 h at room temperature. Then, the sections were washed with PBS 3 times and incubated DAPI (GDP1024, Servicebio, Wuhan, China) and photographed with a microscope.

For lysosensor green dyestain, after 12 h of cell administration, cells were washed three times with PBS, and then cells were stained with lysosomal fluorescent probe (S34855, Gibco, New York, NY, USA) (100 ng/mL) for 5 min photographed with a microscope.