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3D skin model production and treatments

AZ Alessandra Zonari
LB Lear E. Brace
KA Kallie Al-Katib
WP William F. Porto
DF Daniel Foyt
MG Mylieneth Guiang
EC Edgar Andres Ochoa Cruz
BM Bailey Marshall
MG Melissa Gentz
GG Gabriela Rapozo Guimarães
OF Octavio L. Franco
CO Carolina R. Oliveira
MB Mariana Boroni
JC Juliana L. Carvalho
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3D skin models were prepared as described by Maria-Engler et al.64, with minor modifications. Briefly, collagen I gels containing embedded fibroblasts were seeded with keratinocytes and cultured for 24 h. Then, gels were raised to an air-liquid interface and kept for additional 7 days, to allow epidermal cornification. Treatments were performed by adding the molecule in the culture media or with a topical formulation. In 3D skin equivalent assays, Pep 14 was used at 1 μM, and Rapamycin at 100 nM. Treatment was added to the culture media on day 0 and day 3. Topical Pep 14 treatment consisted of 0.01% Pep 14 in a 15% oil moisturizing emulsion. After 5 days, the samples were harvested and fixed in formalin for histology, or used for RNA isolation.

Copyright and License information: The Author(s) ©2023, corrected publication ©2024
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