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Measuring sleep

SS Shashank Shekhar
AM Andrew T. Moehlman
BP Brenden Park
ME Michael Ewnetu
CT Charles Tracy
IT Iris Titos
KP Krzysztof Pawłowski
VT Vincent S. Tagliabracci
HK Helmut Krämer
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CO2 anaesthetized, 3-5 days old male flies were placed inside a 65 ×3 mm glass tube (inside-diameter) with standard molasses fly food on one side and a cotton cover on the other side. Tubes were placed in the Ethoscope arena34,35. Ethoscopes were placed in a 25 °C incubator with 300 Lux light at a 12 h:12 h LD cycle. After allowing flies to acclimatize for one day, their movement was recorded for 4 days. 5-min periods of inactivity, as determined by video tracking34,35 were used to define sleep. Finally, Ethoscope data were analyzed using rethomics34,35. The data shown in the graphs are averages of the 3-day recordings. All codes used in the sleep analysis are available in github (https://github.com/mz27ethio/Ethoscope-Kramerlab.git).

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