Genotyping for the IMAGEN study

CX Chao Xie
SX Shitong Xiang
CS Chun Shen
XP Xuerui Peng
JK Jujiao Kang
YL Yuzhu Li
WC Wei Cheng
SH Shiqi He
MB Marina Bobou
MB M. John Broulidakis
BN Betteke Maria van Noort
ZZ Zuo Zhang
LR Lauren Robinson
NV Nilakshi Vaidya
JW Jeanne Winterer
YZ Yuning Zhang
SK Sinead King
TB Tobias Banaschewski
GB Gareth J. Barker
AB Arun L. W. Bokde
UB Uli Bromberg
CB Christian Büchel
HF Herta Flor
AG Antoine Grigis
HG Hugh Garavan
PG Penny Gowland
AH Andreas Heinz
BI Bernd Ittermann
HL Hervé Lemaître
JM Jean-Luc Martinot
MM Marie-Laure Paillère Martinot
FN Frauke Nees
DO Dimitri Papadopoulos Orfanos
TP Tomáš Paus
LP Luise Poustka
JF Juliane H. Fröhner
US Ulrike Schmidt
JS Julia Sinclair
MS Michael N. Smolka
AS Argyris Stringaris
HW Henrik Walter
RW Robert Whelan
SD Sylvane Desrivières
BS Barbara J. Sahakian
TR Trevor W. Robbins
GS Gunter Schumann
TJ Tianye Jia
JF Jianfeng Feng
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DNA purification and genotyping were performed by the Centre National de Génotypage. DNA was extracted from whole-blood samples (∼10 ml) preserved in BD Vacutainer EDTA Tubes (Becton, Dickinson and Company) using the Gentra Puregene Blood Kit (QIAGEN), according to the manufacturer’s instructions. SNPs with call rates of <98%, minor allele frequency <1% or deviation from the Hardy–Weinberg equilibrium (P < 1.00 × 10−4) were excluded from analyses. Individuals with an ambiguous sex code, excessive missing genotypes (failure rate >2%) and outlying heterozygosity (heterozygosity rate of 3 s.d. from the mean) were also excluded.

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