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Targeted metabolite analysis by LC-MS/MS

JZ Jing Zhou
YL Yijia Liu
YL Yan Li
WL Wenqing Ling
XF Xiaoyu Fan
QF Qixian Feng
RM Ray Ming
FY Fulin Yang
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Before the phytohormone concentration of the samples were tested, a total of 22 kinds of standards were melted with methanol and mixed together to obtain the standard stock solution. Phytohormone metabolites were tested using an ultrahigh-performance liquid phase chromatograph mass spectrometer (UHPLC-Qtrap). The mobile phases were 0.01% formic acid in water (phase A) and 0.01% formic acid in acetonitrile (solvent B). The mass spectral conditions were positive and negative modes, curtain gas of 35 psi, medium collision gas, ion spray voltage floating of +5500/−4500, and source temperature of 550°C.

Copyright and License information:  ©2023 Zhou, Liu, Li, Ling, Fan, Feng, Ming and Yang
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